脂质组学分析

脂质组学是一门近年来发展十分迅速的学科。它可以定义为对脂类分子的大规模的定性和定量的分析,以揭示脂类的功能和在不同生理与病理条件下的变化。脂质组学对脂类生物学和脂类相关的疾病研究的发展作出了巨大的贡献。

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2017年02月25日

蛋白质组学分析

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脂质组学的目标在于建立一个包含整个细胞或组织内的所有脂质的综合脂质图谱,并已成为脂质生物学界、科技行业和医学界的一门独立学科。近期在脂质组学这一研究领域的发展主要是由于分析技术的进步,特别是开发了用于表征和量化细胞脂质体中多样的脂质种类的新型质谱和色谱仪器(Lam SM et al., 2013 and 2017)。在中科脂典,我们提供了多样化的量化脂质分析服务,涵盖了生物体中可能超过10,000种的脂质及其衍生物。

在人类泪液的脂质组学特征的标志性工作中,我们的脂质组学技术的能力是显而易见的。我们关于人类泪液的脂质组学的研究已经刊登在了ASBMB TODAY,同时也在Journal of Lipid Research上发表了一系列的论文(Lam SM et al., 2014, 2014, 2014)。此外,我们在对各种生物流体,包括血清,血浆,尿液,牛奶,以及不同类型的生物组织和生物体(Shui G et al., 2012; Wang W et al., 2012; Chua EC et al., 2013; Nguyen LN et al., 2014)的检测中有着丰富的经验。如果想了解更多信息,请参阅我们关于脂质体服务的介绍视频。凭借我们在脂质研究方面的丰富经验,我们还为感兴趣的客户提供有关选择脂质分析覆盖率的咨询服务以适应需求。


- 磷脂 Phospholipids: PC, PE, PS, PI, PG, PA, CL, LBPA, LPC, LPE, LPS, LPI, LPA  

- 鞘脂 Sphingolipids : SM, Cer, Sph, S1P, GluCer, GalCer, SL, LacCer, GM3, Gb3, etc;

- 固醇 Sterols: cholesterol, cholesteryl esters, cholesterol oxidation products, cholesterol metabolites, cholesterol precursors, other sterols including sitosterol, campestrol, etc; 

- 磷脂酰肌醇磷酸盐类 Phosphatidylinositol phosphates: PIP/PIP2/PIP3 profiles

- N-酰基磷脂酰乙醇氨 NAPE profiles

- 氧化类固醇 Oxysterol profiles, 

- 类固醇和激素 Steroids and hormones

- 游离脂肪酸酰及其衍生物 Free fatty acyls and their derivatives profiles: fatty acids, eicosanoids, etc; 

- 分枝菌酸 Mycolic acid profiles 

- 甘油酯 Glycerol lipids: TAG/DAG/MAG profiles 

- 蜡酯 Wax ester profiles

- 糖脂 Glycolipid profiles: MGDG/DGDG profiles, AC1PIM1/AC1PM2 profiles

- 醌 Quinone profiles

- 乙酰辅酶A Acetyl-CoA, 酰基辅酶a Acyl-CoA profiles

- 肉毒碱 Carnitines, 酰基肉毒碱 Acyl-carnitine profiles



参考文献

Lam SM, Shui G. Lipidomics as a principal tool for advancing biomedical research. Journal of genetics and genomics. 2013; 40(8): 375-90.

Lam SM, Tian H, Shui G. Lipidomics, en route to accurate quantitation. Biochim Biophys Acta. 2017.

Lam SM, Tong L, Duan X, Petznick A, Wenk MR, Shui G. Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles. J Lipid Res. 2014; 55(2): 289-98.

Lam SM, Tong L, Reux B, Duan X, Petznick A, Yong SS, et al. Lipidomic analysis of human tear fluid reveals structure-specific lipid alterations in dry eye syndrome. J Lipid Res. 2014; 55(2): 299-306.

Lam SM, Tong L, Duan X, Acharya UR, Tan JH, Petznick A, et al. Longitudinal changes in tear fluid lipidome brought about by eyelid-warming treatment in a cohort of meibomian gland dysfunction. J Lipid Res. 2014; 55(9):1959-69.

Shui G, Bendt AK, Jappar IA, Lim HM, Laneelle M, Herve M, et al. Mycolic acids as diagnostic markers for tuberculosis case detection in humans and drug efficacy in mice. EMBO Mol Med. 2012; 4(1): 27-37.

Wang W, Lv N, Zhang S, Shui G, Qian H, Zhang J, et al. Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids. Nat Med. 2012;18(2):235-43.

Chua EC, Shui G, Lee IT, Lau P, Tan LC, Yeo SC, et al. Extensive diversity in circadian regulation of plasma lipids and evidence for different circadian metabolic phenotypes in humans. Proc Natl Acad Sci U S A. 2013; 110(35):14468-73.

Nguyen LN, Ma D, Shui G, Wong P, Cazenave-Gassiot A, Zhang X, et al. Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. Nature. 2014;509(7501):503-6.



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